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CyFlow™ CD6 Pacific Blue™

CyFlow™ CD6 Pacific Blue™
Antigen: CD6
Alternative Name: T12
Clone: MEM-98
Application: Flow cytometry
Format/Fluorochrome: Pacific Blue™
Laser: Violet
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 455 nm
Excitation Maximum: 400 to 410 nm
Isotype: IgG1
Order number: BV249149

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 0.4 Immunogen Human CD6 antigen purified by immunoaffinity... more
CyFlow™ CD6 Pacific Blue™
Quantity100 tests
Volume0.4
ImmunogenHuman CD6 antigen purified by immunoaffinity chromatography from HBP-ALL cells followed by preparative SDS-PAGE of non-boiled non-reduced sample (excised piece of gel corresponding to the 100 kDa zone)
Background InformationCD6 (T12) is a member of the scavenger receptor superfamily found on T and B cell subsets, thymocytes, and acute lymphocytic leukemia cells (ALL). CD6 interacts with its ligand CD166 (ALCAM; activated leukocyte cell adhesion molecule) and serves as a coreceptor for T cell activation and stabilizer of the immunological synapse. CD6-ALCAM mediated cell adhesion is also important for T cell proliferation. CD6 may exert some its functions via association with CD5, probably by fine-tuning CD5 signaling. Ligation of CD6 has antiapoptotic role in chronic lymphocytic leukemia B cells.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I: Biochemical characterization of a soluble form of the 53‑kDa monocyte surface antigen. Eur·J·Immunol. 1986·Dec; 16(12):1583‑9. <·PMID:·3493149·> | Osorio LM, De Santiago A, Aguilar-Santelises M, Mellstedt H, Jondal M: CD6 ligation modulates the Bcl‑2/Bax ratio and protects chronic lymphocyticleukemia B cells from apoptosis induced by anti‑IgM. Blood. 1997·Apr·15; 89(8):2833‑41. <·PMID:·9108402·> | Zimmerman AW, Joosten B, Torensma R, Parnes JR, van Leeuwen FN, Figdor CG: Long‑term engagement of CD6 and ALCAM is essential for T‑cell proliferationinduced by dendritic cells. Blood. 2006·Apr·15; 107(8):3212‑20. <·PMID:·16352806·> | Hassan NJ, Simmonds SJ, Clarkson NG, Hanrahan S, Puklavec MJ, Bomb M, Barclay AN, Brown MH: CD6 regulates T‑cell responses through activation‑dependent recruitment of the positive regulator SLP‑76. Mol·Cell·Biol. 2006·Sep; 26(17):6727‑38. <·PMID:·16914752·> | Castro MA, Oliveira MI, Nunes RJ, Fabre S, Barbosa R, Peixoto A, Brown MH, Parnes JR, Bismuth G, Moreira A, Rocha B, Carmo AM: Extracellular isoforms of CD6 generated by alternative splicing regulate targeting of CD6 to the immunological synapse. J·Immunol. 2007·Apr·1; 178(7):4351‑61. <·PMID:·17371992·>