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CyFlow™ CD9 Biotin

CyFlow™ CD9 Biotin
Antigen: CD9
Alternative Name: MRP-1, P24
Clone: MEM-61
Application: Flow cytometry, Western blot
Format/Fluorochrome: Biotin
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Isotype: IgG1
Order number: BE166549

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA VI—WS Code P P-15 Concentration Unit mg/mL Concentration 1... more
CyFlow™ CD9 Biotin
HLDA WorkshopHLDA VI—WS Code P P-15
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1
ImmunogenNALM-6 human pre-B cell line
Background InformationCD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
UsageThe reagent is designed for indirect immunofluorescence analysis by Flow Cytometry and for Western blotting. Suggested working usage is 2.5·µg/ml (for Flow Cytometry). Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Stöckl J, Majdic O, Fischer G, Maurer D, Knapp W: Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA‑A1‑restricted, hapten‑specific CTL. J·Immunol. 2001·Sep·1; 167(5):2724‑33. <·PMID:·11509616·> | Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I: Absence of CD9 enhances adhesion‑dependent morphologic differentiation, survival, and matrix metalloproteinase‑2 production in small cell lung cancer cells. Cancer·Res. 2006·Oct·1; 66(19):9557‑65. <·PMID:·17018612·> | Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc·Natl·Acad·Sci·USA. 2007·Jan·2; 104(1):234‑9. <·PMID:·17190803·> | Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb·Haemost. 2007·Nov; 98(5):1081‑7. <·PMID:·18000614·> | Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose‑derived mesenchymal stem cells. Pflugers·Arch. 2007·Nov; 455(2):283‑96. <·PMID:·17668233·> | Singh AB, Sugimoto K, Dhawan P, Harris RC: Juxtacrine activation of EGFR regulates claudin expression and increases transepithelial resistance. Am·J·Physiol·Cell·Physiol. 2007·Nov; 293(5):C1660‑8. <·PMID:·17855771·> | Lafleur MA, Xu D, Hemler ME: Tetraspanin proteins regulate membrane type‑1 matrix metalloproteinase‑dependent pericellular proteolysis. Mol·Biol·Cell. 2009·Apr; 20(7):2030‑40. <·PMID:·19211836·>