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CyFlow™ CD21 Pacific Blue™

CyFlow™ CD21 Pacific Blue™
Antigen: CD21
Alternative Name: C3dR, CR2, EBV-R
Clone: LT21
Application: Flow cytometry
Format/Fluorochrome: Pacific Blue™
Laser: Violet
Target Species: Cow, Dog, Human, Pig
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 455 nm
Excitation Maximum: 400 to 410 nm
Isotype: IgG1
Order number: BZ161140

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

HLDA Workshop HLDA VI—WS Code B CD21.1 Quantity 100 tests Volume 0.4 Immunogen... more
CyFlow™ CD21 Pacific Blue™
HLDA WorkshopHLDA VI—WS Code B CD21.1
Quantity100 tests
Volume0.4
ImmunogenIM9 human B-lymphoblastoid cell line
Background InformationCD21 (CR2; complement receptor 2) binds C3 complement fragments, especially its breakdown fragments, which remain covalently attached to complement activating surfaces or antigen. CD21 has important roles in uptake and retention of immunocomplexes, survival of memory B cells and in development and maintenance of the humoral response to T-dependent antigens. CD21 also serves as a key receptor for Epstein-Barr virus binding and is involved in targeting prions to folicular dendritic cells and expediting neuroinvasion following peripheral exposure to prions. A soluble form of the CD21 (sCD21) is shed from the lymphocyte surface and retains its ability to bind respective ligands.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

Specific References

| Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·> | Kasprzak A, Spachacz R, Wachowiak J, Stefanska K, Zabel M: Epstein‑Barr virus (EBV) infection in B‑cell non‑Hodgkin's lymphomas in children: virus latency and its correlation with CD21 and CD23 molecules. Folia·Histochem·Cytobiol. 2007; 45(3):169‑79. <·PMID:·17951165·> | Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass‑spectrometry. J·Immunol·Methods. 2007·Jan·30; 319(1‑2):21‑33. <·PMID:·17098248·> | Twohig J, Kulik L, Haluszczak C, Reuter J, Rossbach A, Bull M, Holers VM, Marchbank KJ: Defective B cell ontogeny and immune response in human complement receptor 2 (CR2, CD21 transgenic mice is partially recovered in the absence of C3. Mol·Immunol. 2007·Jul; 44(13):3434‑44. <·PMID:·17379312·> | Faldyna M, Samankova P, Leva L, Cerny J, Oujezdska J, Rehakova Z, Sinkora J: Cross‑reactive anti‑human monoclonal antibodies as a tool for B‑cell identification in dogs and pigs. Vet·Immunol·Immunopathol. 2007·Sep·15; 119(1‑2):56‑62. <·PMID:·17673300·> | Roozendaal R, Carroll MC: Complement receptors CD21 and CD35 in humoral immunity. Immunol·Rev. 2007·Oct; 219:157‑66. <·PMID:·17850488·> | Zabel MD, Heikenwalder M, Prinz M, Arrighi I, Schwarz P, Kranich J, von Teichman A, Haas KM, Zeller N, Tedder TF, Weis JH, Aguzzi A: Stromal complement receptor CD21/35 facilitates lymphoid prion colonization and pathogenesis. J·Immunol. 2007·Nov·1; 179(9):6144‑52. <·PMID:·17947689·> | Singh A, Blank M, Shoenfeld Y, Illges H: Antiphospholipid syndrome patients display reduced titers of soluble CD21 in their sera irrespective of circulating anti‑beta2‑glycoprotein‑I autoantibodies. Rheumatol·Int. 2008·Jan·3; <·PMID:·23949624·>