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CyFlow™ CD25 PerCP

CyFlow™ CD25 PerCP
Antigen: CD25
Alternative Name: IL-2R, p55, TAC
Clone: MEM-181
Application: Flow cytometry
Format/Fluorochrome: PerCP
Laser: Blue, Green
Target Species: Human
Field of Interest: Immunophenotyping
Species of Origin: Mouse
Regulatory Status: RUO
Clonality: monoclonal
Emission Maximum: 678 nm
Excitation Maximum: 482 to 490 nm
Isotype: IgG1
Order number: BH857628

For Research Use Only
Not for use in diagnostic or therapeutic procedures.

Quantity 100 tests Volume 1 ml Immunogen PHA-activated peripheral blood leucocytes... more
CyFlow™ CD25 PerCP
Quantity100 tests
Volume1 ml
ImmunogenPHA-activated peripheral blood leucocytes
SpecifityThe mouse monoclonal antibody MEM-181 recognizes CD25 antigen, a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
Background InformationCD25 (IL2Rα, Tac) is a ligand-binding α subunit of interleukin 2 receptor (IL2R). Together with β and γ subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.
UsageThe reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10·µl reagent·/ 100·µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.1% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.

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