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CyFlow™ CD25 Purified

CyFlow™ CD25 Purified
Application: Flow cytometry, Functional assays, Immunohistochemistry (frozen sections), Immunoprecipitation
Field of Interest: Immunophenotyping, MHC
Alternative Name: IL2Rα, p55, TAC
Antigen: CD25
Source Species: Rat
Target Species: Mouse
Clonality: monoclonal
Isotype: IgG1
Format/Fluorochrome: Purified
Regulatory Status: RUO
Clone: PC61.5
Order number: CN509036
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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
Product information "CyFlow™ CD25 Purified"
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenB6.1 CTL cell line
Background InformationCD25 (IL2Rα, Tac) is a ligand-binding α subunit of interleukin 2 receptor (IL2R). Together with β and γ subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. CD25+ CD4+ FoxP3+ regulatory cells (Treg cells) play a crucial role in the control of organ-specific autoimmune diseases.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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* All prices are quoted net of the statutory value-added tax and shipping costs and possibly delivery charges, if not otherwise described

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