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CyFlow™ CD15 Pacific Blue™

CyFlow™ CD15 Pacific Blue™
Application: Flow cytometry
Isotype: IgM
Alternative Name: 3-fucosyl-N-acetyllactosamine, Le(x), Lewis X, SSEA-1
Field of Interest: Blood group antigens, Immunophenotyping
Clone: MEM-158
Antigen: CD15
Emission Maximum: 455 nm
Clonality: monoclonal
Excitation Maximum: 400 to 410 nm
Laser: Violet
Format/Fluorochrome: Pacific Blue™
Source Species: Mouse
Regulatory Status: RUO
Target Species: Human
Order number: BS939780
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HLDA Workshop HLDA VI - WS Code AS A053 Quantity 100 tests Volume 1.0 mL Immunogen... more
Product information "CyFlow™ CD15 Pacific Blue™"
HLDA WorkshopHLDA VI - WS Code AS A053
Quantity100 tests
Volume1.0 mL
ImmunogenHuman granulocytes
Background InformationCD15 (Le(x); Lewis x or SSEA-1; stage specific embryonic antigen-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumor cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
UsageThe reagent is designed for flow cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Storage BufferThe reagent is provided in stabilizing MES buffered saline solution, pH ≈5.5, containing 0.09% (w/v) sodium azide and 0.2% (w/v) BSA.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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