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CyFlow™ ARHGEF4 Purified

CyFlow™ ARHGEF4 Purified
Application: Flow cytometry, Western blot
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Format/Fluorochrome: Purified
Isotype: IgG1
Field of Interest: Signaling
Antigen: ARHGEF4
Clone: ARHGEF-08
Order number: CC233224
159,28  EUR *

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
Product information "CyFlow™ ARHGEF4 Purified"
Application: Flow cytometry, Western blot
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Source Species: Mouse
Format/Fluorochrome: Purified
Isotype: IgG1
Field of Interest: Signaling
Antigen: ARHGEF4
Clone: ARHGEF-08
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenRecombinant fragment of human ARHGEF4 (amino acids 143-271)
Background InformationARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF1 (Adenomatous polyposis coli - stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), Pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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